Dataset Information

FlashPack: Fast and simple preparation of ultra-high performance capillary columns for LC-MS

ABSTRACT: High-resolution capillary chromatography is essential for in-depth characterization of complex biomolecule mixtures by LC-MS. We report a simple and low-cost FlashPack protocol for preparation of ultra-high performance capillary columns of > 50 cm length in less than 1 h using low-pressure equipment. Flashpack LC columns enable robust identification of >35,000 unique peptides and 5,800 human proteins in a single LC-MS analysis.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Myotube, Fibroblast

SUBMITTER: Adelina Rogowska-Wrzesinska

LAB HEAD: Adelina Rogowska-Wrzesinska

PROVIDER: PXD009519 | Pride | 2019-01-21

REPOSITORIES: Pride

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Publications

FlashPack: Fast and Simple Preparation of Ultrahigh-performance Capillary Columns for LC-MS.

Kovalchuk Sergey I SI Jensen Ole N ON Rogowska-Wrzesinska Adelina A

Molecular & cellular proteomics : MCP 20181029 2

Capillary ultrahigh-pressure liquid chromatography (cUHPLC) is essential for in-depth characterization of complex biomolecule mixtures by LC-MS. We developed a simple and fast method called <i>FlashPack</i> for custom packing of capillary columns of 50-100 cm length with sub- 2 μm sorbent particles. <i>FlashPack</i> uses high sorbent concentrations of 500-1,000 mg/ml for packing at relatively low pressure of 100 bar. Column blocking by sorbent aggregation is avoided during the packing by gentle ...[more]

PMID: 30373789

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Dataset Information

FlashPack: Fast and simple preparation of ultra-high performance capillary columns for LC-MS

Publications

FlashPack: Fast and Simple Preparation of Ultrahigh-performance Capillary Columns for LC-MS.

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