FlashPack: Fast and simple preparation of ultra-high performance capillary columns for LC-MS

ABSTRACT: High-resolution capillary chromatography is essential for in-depth characterization of complex biomolecule mixtures by LC-MS. We report a simple and low-cost FlashPack protocol for preparation of ultra-high performance capillary columns of > 50 cm length in less than 1 h using low-pressure equipment. Flashpack LC columns enable robust identification of >35,000 unique peptides and 5,800 human proteins in a single LC-MS analysis.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Myotube, Fibroblast

SUBMITTER: Adelina Rogowska-Wrzesinska  

LAB HEAD: Adelina Rogowska-Wrzesinska

PROVIDER: PXD009519 | Pride | 2019-01-21

REPOSITORIES: Pride