Proteomics

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Conformational control and DNA binding mechanism of the metazoan origin recognition complex

ABSTRACT: Chemical cross-linking coupled to mass spectrometry was used to study the architecture and conformational state(s) of the Drosophila melanogaster origin replication complex (ORC). Two versions of the ORC were subjected to cross-linking with the amine-reactive reagents, disuccinimidyl suberate (DSS). One was a complex with truncated subunits (“core complex”), one with full-length sequences of all six subunits (“full-length complex”).

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

SUBMITTER: Alexander Leitner  

LAB HEAD: Alexander Leitner

PROVIDER: PXD009526 | Pride | 2018-06-11

REPOSITORIES: Pride

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Publications

Conformational control and DNA-binding mechanism of the metazoan origin recognition complex.

Bleichert Franziska F   Leitner Alexander A   Aebersold Ruedi R   Botchan Michael R MR   Berger James M JM  

Proceedings of the National Academy of Sciences of the United States of America 20180613 26

In eukaryotes, the heterohexameric origin recognition complex (ORC) coordinates replication onset by facilitating the recruitment and loading of the minichromosome maintenance 2-7 (Mcm2-7) replicative helicase onto DNA to license origins. <i>Drosophila</i> ORC can adopt an autoinhibited configuration that is predicted to prevent Mcm2-7 loading; how the complex is activated and whether other ORC homologs can assume this state are not known. Using chemical cross-linking and mass spectrometry, bioc  ...[more]

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