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Time-resolved phosphoproteomic analysis of ZIKV-infected cells

ABSTRACT: To investigate cellular pathway dysregulation upon ZIKV infection, time-resolved phosphoproteomics LC-MS/MS analysis was used. ZIKV-infected SK-N-BE2 cells or mock-treated cells were harvested 24, 48 and 72 hours post virus infection and their phosphoproteome was quantitatively analyzed by label-free LC-MS/MS.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Zika Virus Zikv/h. Sapiens/frenchpolynesia/10087pf/2013 Homo Sapiens (human)

TISSUE(S): Cell Culture, Neuroblast

SUBMITTER: Pietro Scaturro

LAB HEAD: Andreas Pichlmair

PROVIDER: PXD009560 | Pride | 2018-09-03

REPOSITORIES: Pride

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An orthogonal proteomic survey uncovers novel Zika virus host factors.

Scaturro Pietro P Stukalov Alexey A Haas Darya A DA Cortese Mirko M Draganova Kalina K Płaszczyca Anna A Bartenschlager Ralf R Götz Magdalena M Pichlmair Andreas A

Nature 20180903 7722

Zika virus (ZIKV) has recently emerged as a global health concern owing to its widespread diffusion and its association with severe neurological symptoms and microcephaly in newborns<sup>1</sup>. However, the molecular mechanisms that are responsible for the pathogenicity of ZIKV remain largely unknown. Here we use human neural progenitor cells and the neuronal cell line SK-N-BE2 in an integrated proteomics approach to characterize the cellular responses to viral infection at the proteome and ph ...[more]

PMID: 30177828

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