Proteome allocation strategies under nutrient-limiting conditions in the cyanobacterium Synechocystis

ABSTRACT: The response of the model cyanobacterium Synechocystis sp. PCC6803 towards light and carbon limitation was systematically probed. To this end, Synechocystis sp. PCC6803 was cultivated in a photo-bioreactor driven in turbidostat-mode. The turbidostat is a continuous cultivation that enabled cells to adapt to a constant environment, leading to a stable and 'optimal' proteome for the respective condition. The major dataset in this project consisted of 5 different 'concentrations' for light and CO2. Changes in the proteome were determined using using liquid chromatography/mass spectrometry and it was found that carbon and light limitation induced gradual but broad responses in gene expression. With decreasing substrate concentration (increasing limitation) a decrease in growth rate and a gradually more severe response in the proteome was visible.

INSTRUMENT(S):

ORGANISM(S): Synechocystis Sp. Pcc 6803 Substr. Gt-s

TISSUE(S): Photosynthetic Cell

DISEASE(S): Disease Free

SUBMITTER: Michael Jahn  

LAB HEAD: Elton Paul Hudson

PROVIDER: PXD009582 | Pride | 2018-09-12

REPOSITORIES: Pride