Toxoplasma GRA protein identification
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q-Tof ultima
ORGANISM(S): Toxoplasma
TISSUE(S): Cell Culture, Fibroblast
DISEASE(S): Toxoplasmosis
SUBMITTER: 
Bang Shen
LAB HEAD: Bang Shen
PROVIDER: PXD009604 | Pride | 2018-11-02
REPOSITORIES: Pride
Publications
Identification of Novel Dense-Granule Proteins in Toxoplasma gondii by Two Proximity-Based Biotinylation Approaches.
Pan Ming M Li Mingjun M Li Longjiao L Song Yongle Y Hou Lun L Zhao Junlong J Shen Bang B
Journal of proteome research 20181026 1
Toxoplasma gondii is an opportunistic pathogen infecting humans and a variety of vertebrate animals. Secretory dense-granule proteins (GRAs) play diverse roles in the mediation of host-parasite interactions and facilitate parasitism, but many of them still remain to be identified. Here, we used two proximity-based protein labeling techniques to identify novel GRA proteins. Taking GRA1 as bait, transgenic strains expressing GRA1-BirA* or GRA1-APEX were constructed to biotinylate GRAs. Using these ...[more]
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