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In-situ Click Reaction Coupled with Quantitative Proteomics for Identification of Protein Targets of Catechol Estrogens

ABSTRACT: Catechol estrogens (CEs) are metabolic electrophiles that actively undergo covalent interaction with cellular proteins, influencing molecular function. There is no feasible method to identify their binders in a living system. Herein, we developed a click chemistry-based approach using ethinylestradiol(EE2) as the precursor probe coupled with quantitative proteomics to identify protein targets of CEs and classify their binding strengths.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Liver

DISEASE(S): Breast Cancer

SUBMITTER: Yi-Chen Liu

LAB HEAD: Chen, Shu-Hui

PROVIDER: PXD009657 | Pride | 2022-02-28

REPOSITORIES: Pride

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