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Improving silkworm genome annotation using a proteogenomics approach

ABSTRACT: We collected a total of 9.8 million mass spectra data generated in the laboratory from the proteomics analyses of different silkworm tissues, including the posterior silk gland 29, middle silk gland 30, ovary and testis 31, head 32, brain, prothoracic glands, subesophageal ganglion 33, hemolymph 34, fat body 35 and embryo 36,37 of domestic silkworm, and the posterior silk gland of wild silkworm 38.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Bombyx Mori (silk Moth)

TISSUE(S): Head

SUBMITTER: Boxiong Zhong

LAB HEAD: Bo-xiong Zhong

PROVIDER: PXD009672 | Pride | 2019-07-01

REPOSITORIES: Pride

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Improving Silkworm Genome Annotation Using a Proteogenomics Approach.

Ye Xiaogang X Tang Xiaoli X Wang Xiaoxiao X Che Jiaqian J Wu Meiyu M Liang Jianshe J Ye Lupeng L Qian Qiujie Q Li Jianying J You Zhengying Z Zhang Yuyu Y Wang Shaohua S Zhong Boxiong B

Journal of proteome research 20190702 8

The silkworm genome has been deeply sequenced and assembled, but accurate genome annotation, which is important for modern biological research, remains far from complete. To improve silkworm genome annotation, we carried out a proteogenomics analysis using 9.8 million mass spectra collected from different tissues and developmental stages of the silkworm. The results confirmed the translational products of 4307 existing gene models and identified 1701 novel genome search-specific peptides (GSSPs) ...[more]

PMID: 31250652

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The proteomics analyses of different tissues(the posterior silk gland of wild silkworm)

Project description:We collected a total of 9.8 million mass spectra data generated in the laboratory from the proteomics analyses of different silkworm tissues, including the posterior silk gland (29), middle silk gland (30), ovary and testis (31), head (32), brain, prothoracic glands, subesophageal ganglion (33), hemolymph (34), fat body (35) and embryo (36,37) of domestic silkworm, and the posterior silk gland of wild silkworm (38).

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The proteomics analyses of different silkworm tissues (middle silk gland and ovary)