Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

GRX deleted Saccharomyces Cerevisiae LC-MSMS

ABSTRACT: In this study, we used Saccharomyces cerevisiae to investigate the effects of GRX deletion on yeast chronological life span (CLS). Deletion of Grx1 or Grx2 shortened yeast CLS. Quantitative proteomics revealed that GRX deletion increased cellular ROS levels to activate Ras/PKA signal pathway. Our results provided new insights into mechanisms underlying aging process.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Yan Liu

LAB HEAD: Haiteng Deng

PROVIDER: PXD009699 | Pride | 2022-02-25

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Similar Datasets

Significant and Systematic Expression Differentiation in Long-Lived Yeast Strains

Project description:The three yeast mutants sch9Δ, ras2Δ, tor1Δ show extended chronological life span up to three folds. This study aims to dissect the mechanisms that lead to the yeast life span extension. Keywords: Expression comparison between wild type and long-lived yeast mutant strain

2008-11-01 | GSE13420 | GEO

Wine yeast EC1118 and CSM comparative transcriptomics during synthetic grape juice fermentation MS300 at day 6

2011-10-26 | GSE33068 | GEO

Bre1-mediated Set2 protein degradation promotes chronological aging in budding yeast [RNA-seq]

Project description:Histone modification affects life span in various organisms. The loss of Histone H3K36 methylation can shorten replicative life span in Saccharomyces cerevisiae. However, budding yeast, as a model organism for aging research, has replicative life span (RLS) and chronological life span (CLS). In this study, we showed that the loss of Histone H3K36 methylation can shorten CLS in Saccharomyces cerevisiae. We identified Ubc3/Bre1 mediates polyubiquitination of Set2 K25 and K530 at log phase and stationary phase, and Bre1 interacts with Ubc3 and Rad6 simultaneously. BRE1 knockout can stabilize Set2 protein to maintain H3K36me3 and regulate the transcription of aging related genes, such as DSE1/DSE2/SUN4/EGT2/SCW11. We also proved that Gcn5-mediated Set2 acetylation regulates Set2 protein stability and chronological aging. Altogether, our study showed that knockout of BRE1 and GCN5 regulate Set2 protein level by mediating the polyubiquitination of Set2 to influence the level of H3K36me3 and the transcription level of aging related genes enriched by H3K36me3, thereby extending the chronological life span.

2023-09-29 | GSE221025 | GEO

Bre1-mediated Set2 protein degradation promotes chronological aging in budding yeast [ChIP-seq]

2023-09-29 | GSE221024 | GEO

Wine yeast EC1118 and CSM comparative transcriptomics during synthetic grape juice fermentation MS300 at day 6

Project description:CSM is a commercial wine yeast with a long chronological life span, while commercial strain EC1118 displays a short life span in laboratory synthetic complete medium SC. Our aim is compare their expression profile on a high sugar fermentation From a preculture of two days in YPD, MS300 was innoculated at 10exp6 cells/mL in filled-in 50 mL conical centrifuge tubes and they were cultivated at 24ºC with mild shaking for 6 days.

2011-10-25 | E-GEOD-33068 | biostudies-arrayexpress

Comparative genomic hybridization by array of east haploid and heterozygous essential deletion pools treated with compounds synergistic with fluconazole to identify sensitive and resistant mutants

Project description:The haploid and the heterozygous essential S.cerevisiae deletion pools were grown in the presence of compounds that we found to be synergistic with fluconazole. We also treated the deletion pools with combinations of those drugs and fluconazole to interrogate the mechanism of action of the drug interactions. These experimental samples were hybridized against DMSO or water control samples. The resulting hybridization pattern informs about sensitive and resistant yeast deletion mutants.

2011-09-07 | E-MTAB-394 | biostudies-arrayexpress

Mutant2

Project description:Cells in glucose-limited Saccharomyces cerevisiae cultures differentiate into quiescent (Q) and non-quiescent (NQ) fractions prior to entering stationary phase. To identify genes involved in this differentiation, Q and NQ cells from 101 deletion-mutant strains were tested for viability and reproductive capacity. Twenty-one mutants were identified, including 7 that affected reproductive capacity of both cell types. Thirteen affected only Q or NQ cells, indicating significant differentiation of these cell types. doa4 strains, lacking ubiquitin hydrolase, affected viability and reproductive capacity in both cell types. More than 1300 mRNAs differentiating Q and NQ cell fractions were identified by microarray analysis. Gene-ontology analysis of Q-cell mRNAs showed significant increases in protein-encoding mRNAs involved in membrane maintenance, oxidative stress response, and signal transduction. NQ-cell mRNAs encode proteins involved in Ty-element transposition and DNA recombination, consistent with apoptosis in these cells. Consistent with preparation for rapid response to environmental stimuli, approximately 2000 protease-labile mRNAs were identified in Q cells. The differentiation of these cell types and the ability of genes to selectively affect the survival of Q or NQ cells in yeast are relevant to chronological aging, cell-cycle, genome-evolution, and stem-cell research and provides insight into complex responses that even simple organisms have to starvation. Yeast deletion mutants (BY4742 background) were grown to stationary-phase (7 days) and then separted into Q and NQ cells using Percoll density gradients. Microarrays were carried out on these different cell fractions.

2007-12-26 | E-GEOD-8561 | biostudies-arrayexpress

Mutant1

2007-12-26 | E-GEOD-8560 | biostudies-arrayexpress

Project description:yeast chronological aging

| PRJNA544860 | ENA

Transcription profiling of S. cerevisiae (budding yeast) mutants reveals Isc1p plays a key role in hydrogen peroxide resistance and chronological lifespan through modulation of iron levels and programmed cell death

Project description:Changes in the transcriptome of Isc1p deficient mutants. The inositolphosphosphingolipid phospholipase C (Isc1p) of Saccharomyces cerevisiae belongs to the family of neutral sphingomyelinases that generates the bioactive sphingolipid ceramide. In this work the role of Isc1p in oxidative stress resistance and chronological lifespan was investigated. Loss of Isc1p resulted in a higher sensitivity to hydrogen peroxide that was associated with an increase in oxidative stress markers, namely intracellular oxidation, protein carbonylation and lipid peroxidation. Microarray analysis showed that Isc1p deficiency upregulated the iron regulon leading to increased levels of iron, which is known to catalyze the production of the highly reactive hydroxyl radicals via the Fenton reaction. In agreement, iron chelation suppressed hydrogen peroxide sensitivity of isc1D mutants. Cells lacking Isc1p also displayed a shortened chronological lifespan associated with oxidative stress markers and ageing of parental cells was correlated with a decrease in Isc1p activity. The analysis of DNA fragmentation and caspase activity showed that Isc1p deficiency increased apoptotic programmed cell death associated with oxidative stress and ageing. Furthermore, deletion of Yca1p metacaspase suppressed the oxidative stress sensitivity and premature ageing phenotypes of isc1D mutants. These results indicate that Isc1p plays an important role in the regulation of cellular redox homeostasis, through modulation of iron levels, and of apoptosis.

2008-02-05 | E-MEXP-1136 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data