Dataset Information

Fingermark Proteomics - Proteomics as a new tool to study fingermark ageing in forensics

ABSTRACT: Fingermarks are frequently encountered at crime scenes and on related items, and naturally protected against fast degradation due to the abundance of recalcitrant substances, such as fatty acids and constituents of sebum. While representing a major and valuable source for donor identification through latent fingerprint analysis, fingermarks are also rich in molecules that can be used to acquire toxicological and biological donor profiling information. In addition, exogenous compounds in fingermarks have shown applicability for crime reconstruction and to assess offender and victim activities before, during and after a criminal act. A major limitation in these respects is the lack of temporal information, which makes it difficult to establish forensic timelines. To address this issue, we developed a fingermark sampling and protein extraction protocol easily adaptable to forensic settings. A bottom-up proteomics approach was then applied to confidently identify protein traces and assess the fingermark proteome dynamic changes at different stages of ageing. This approach was also applied to contaminated fingermarks to examine fingermark ageing in light of realistic forensic scenarios and detect donor contact with bodily fluids. These data represent HR-MS recordings from fingermarks deposited on glass substrate. Fingermarks originated from both female and male donors and from all five digits, and were aged for up to 16 days. Contamination sources included urine, saliva and vaginal fluid.

INSTRUMENT(S): 6520 Quadrupole Time-of-Flight LC/MS

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Stijn Oonk

LAB HEAD: Stijn Oonk

PROVIDER: PXD009706 | Pride | 2018-12-21

REPOSITORIES: Pride

ACCESS DATA

Publications

Proteomics as a new tool to study fingermark ageing in forensics.

Oonk Stijn S Schuurmans Tom T Pabst Martin M de Smet Louis C P M LCPM de Puit Marcel M

Scientific reports 20181106 1

Fingermarks are trace evidence of great forensic importance, and their omnipresence makes them pivotal in crime investigation. Police and law enforcement authorities have exploited fingermarks primarily for personal identification, but crucial knowledge on when fingermarks were deposited is often lacking, thereby hindering crime reconstruction. Biomolecular constituents of fingermark residue, such as amino acids, lipids and proteins, may provide excellent means for fingermark age determination, ...[more]

PMID: 30401937

Similar Datasets

Project description:Integrity of mitochondrial DNA (mtDNA), encoding several subunits of the respiratory chain, is essential to maintain mitochondrial fitness. Mitochondria, as a central hub for metabolism, are affected in a wide variety of human diseases but also during normal ageing, where mtDNA integrity is compromised. Mitochondrial quality control mechanisms work at different levels, and mitophagy and its variants are critical to remove dysfunctional mitochondria and mtDNA to maintain cellular homeostasis. Understanding the mechanisms governing a selective turnover of mutation-bearing mtDNA without affecting the entire mitochondrial pool is fundamental to design therapeutic strategies against mtDNA diseases and ageing. Here, we show that mtDNA damage after expressing a dominant negative version of the mitochondrial helicase Twinkle, or by chemical means, leads to an exacerbated mtDNA turnover. mtDNA removal depends on lysosomal function and requires the autophagy protein Atg5 but is independent of canonical mitophagy or autophagy. Using proximity labelling, we demonstrated that the area of influence of mitochondrial nucleoids differs upon mtDNA damage, which induces mitochondrial membrane remodelling and endosomal recruitment in close proximity to mitochondrial nucleoid sub compartments. Targeting of nucleoids is controlled by the mitochondrial transmembrane proteins ATAD3 and SAMM50, which together with the endosomal trafficking protein VPS35, orchestrate endosomal nucleoid engulfment. SAMM50 acts as a gatekeeper to avoid mtDNA release to the cytoplasm and facilitating mtDNA transfer to VPS35. Lastly, we show that stimulation of lysosomal activity by rapamycin selectively removes mtDNA deletions in vivo, without affecting mtDNA copy number. With these results, we unveil the molecular players of a new complex mechanism specifically targeting and removing mutant mtDNA which occurs outside the mitochondrial network, a process with multiple potential benefits to understand human mtDNA related diseases, either inherited, acquired or due to normal ageing.

Dataset Information

Fingermark Proteomics - Proteomics as a new tool to study fingermark ageing in forensics

Publications

Proteomics as a new tool to study fingermark ageing in forensics.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure