PROMIS, global analysis of PROtein-Metabolite Interactions using Size separation in Arabidopsis thaliana

ABSTRACT: Herein, we describe a method, dubbed PROMIS that allows simultaneous, global analysis of endogenous protein–small-molecule and protein-protein complexes. To this end a cell-free native lysate was subjected to size-exclusion chromatography followed by quantitative metabolomic and proteomic analysis. Applying this approach to an extract from Arabidopsis thaliana cell cultures, we could retrieve known protein-protein (PPI) and protein–metabolite (PMI) interactions, validating our strategy.

OTHER RELATED OMICS DATASETS IN: MTBLS94

INSTRUMENT(S):

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Cell Culture

SUBMITTER: Michal Gorka  

LAB HEAD: Aleksandra Skirycz

PROVIDER: PXD009714 | Pride | 2018-06-06

REPOSITORIES: Pride