Human testis off-line LC-MS/MS
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive HF-X
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Testis
SUBMITTER: 
Yihao Wang
LAB HEAD: Ping Xu
PROVIDER: PXD009737 | Pride | 2018-10-04
REPOSITORIES: Pride
Publications
Multiproteases Combined with High-pH Reverse-Phase Separation Strategy Verified Fourteen Missing Proteins in Human Testis Tissue.
Sun Jinshuai J Shi Jiahui J Wang Yihao Y Chen Yang Y Li Yanchang Y Kong Degang D Chang Lei L Liu Fengsong F Lv Zhitang Z Zhou Yue Y He Fuchu F Zhang Yao Y Xu Ping P
Journal of proteome research 20181016 12
Subsequent to conducting the Chromosome-Centric Human Proteome Project, we have focused on human testis-enriched missing proteins (MPs) since 2015. For protein coverage to be enhanced, a multiprotease strategy was used for separation of samples by 10% SDS-PAGE. For the separating efficiency to be improved, a high-pH reverse phase (RP) separation strategy was applied to fractionate complex samples in this study. A total of 11,558 proteins was identified, which is the largest proteome data set for ...[more]
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