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Arabidopsis mutant proteomics assay

ABSTRACT: We had a mutant that might be affected for certain protein translation. Triplicate samples were taken from mutant and wild type, each labeled individually for iTRAQ labels. Plant were taken at early vegetative grwoth state for total protein extraction and fractionation, followed by trypsin digestion and peptide analysis using LC-MS/MS.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Whole Body, Leaf

SUBMITTER: Peng Chen

LAB HEAD: Peng Chen

PROVIDER: PXD009770 | Pride | 2019-11-12

REPOSITORIES: Pride

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AtTrm5a catalyses 1-methylguanosine and 1-methylinosine formation on tRNAs and is important for vegetative and reproductive growth in Arabidopsis thaliana.

Jin Xiaohuan X Lv Zhengyi Z Gao Junbao J Zhang Rui R Zheng Ting T Yin Ping P Li Dongqin D Peng Liangcai L Cao Xintao X Qin Yan Y Persson Staffan S Zheng Bo B Chen Peng P

Nucleic acids research 20190101 2

Modified nucleosides on tRNA are critical for decoding processes and protein translation. tRNAs can be modified through 1-methylguanosine (m1G) on position 37; a function mediated by Trm5 homologs. We show that AtTRM5a (At3g56120) is a Trm5 ortholog in Arabidopsis thaliana. AtTrm5a is localized to the nucleus and its function for m1G and m1I methylation was confirmed by mutant analysis, yeast complementation, m1G nucleoside level on single tRNA, and tRNA in vitro methylation. Arabidopsis attrm5a ...[more]

PMID: 30508117

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