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SWATH-MS analysis of 3-hydroxy-3-methylglutaryl-CoA synthase specific inhibitor (F-244)-treated Arabidopsis

ABSTRACT: 3-Hydroxy-3-methylglutaryl-CoA synthase (HMGS) is the second enzyme in the mevalonate pathway. F-244 is the specific inhibitor of HMGS. F-244 treatment caused reduced primary root growth. SWATH-MS quantitative proteomics analysis was carried out to identify differential expressed proteins in F-244-treated primary roots.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Root

SUBMITTER: Pan Liao

LAB HEAD: Mee-Len Chye

PROVIDER: PXD009776 | Pride | 2019-10-01

REPOSITORIES: Pride

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Overexpression of HMG-CoA synthase promotes Arabidopsis root growth and adversely affects glucosinolate biosynthesis.

Liao Pan P Lung Shiu-Cheung SC Chan Wai Lung WL Bach Thomas J TJ Lo Clive C Chye Mee-Len ML

Journal of experimental botany 20200101 1

3-Hydroxy-3-methylglutaryl-CoA synthase (HMGS) catalyses the second step of the mevalonate (MVA) pathway. An HMGS inhibitor (F-244) has been reported to retard growth in wheat, tobacco, and Brassica juncea, but the mechanism remains unknown. Although the effects of HMGS on downstream isoprenoid metabolites have been extensively reported, not much is known on how it might affect non-isoprenoid metabolic pathways. Here, the mechanism of F-244-mediated inhibition of primary root growth in Arabidops ...[more]

PMID: 31557302

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