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Generation of a C3a-specific monoclonal antibody and immunoapheresis of C3a/C3 and its associates

ABSTRACT: A high affine murine monoclonal antibody was generated against complement factor C3 and selected for binding to the C3a region to serve as immunoaffinity reagent. Subsequently, a C3a/C3 specific immunoaffinity column was developed and apheresis of C3a/C3 and its associates was performed. A proteomic analysis was carried out for identification of apheresis products.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Serum

SUBMITTER: Richard Imre

LAB HEAD: Ludwig Wagner

PROVIDER: PXD009829 | Pride | 2019-11-27

REPOSITORIES: Pride

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Intracellular activation of complement C3 leads to PD-L1 antibody treatment resistance via modulating tumor-associated macrophages

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