Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)
TISSUE(S): Root
SUBMITTER: Yanmei Chen
LAB HEAD: Yanmei Chen
PROVIDER: PXD009836 | Pride | 2022-03-02
REPOSITORIES: Pride
Chen Yanmei Y Hoehenwarter Wolfgang W
The Plant journal : for cell and molecular biology 20190211 2
Mass spectrometry has been instrumental in enabling the study of molecular signaling on a cellular scale by way of site-specific quantification of protein post-translational modifications, in particular phosphorylation. Here we describe an updated tandem metal oxide affinity chromatography (MOAC) combined phosphoprotein/phosphopeptide enrichment strategy, a scalable phosphoproteomics approach that allows rapid identification of thousands of phosphopeptides in plant materials. We implemented modi ...[more]