Proteomics

Dataset Information

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Aluminum and salt induced protein changes in tomato plants transformed with bar gene


ABSTRACT: In this project, seedlings of tomato (Solanum lycopersicum) ‘Money Maker’ plants transformed with a bar gene were treated under 20 µM Al3+ and salt (200 mM NaCl) for two weeks. The treatments were conducted in hydroponic solutions in a greenhouse at 24±2C without supplemental light. Roots harvested were immediately frozen in liquid nitrogen and stored at -80 oC. The treatment experiment was conducted at Agricultural Research Station, Tennessee State University, Nashville, TN 37209

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Solanum Lycopersicum

TISSUE(S): Plant Cell, Root

SUBMITTER: Ted Thannhauser  

LAB HEAD: Theodore Thannhauser

PROVIDER: PXD009848 | Pride | 2020-04-09

REPOSITORIES: Pride

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Publications


Glyoxalase I (Gly I) is the first enzyme in the glutathionine-dependent glyoxalase pathway for detoxification of methylglyoxal (MG) under stress conditions. Transgenic tomato 'Money Maker' plants overexpressing tomato <i>SlGlyI</i> gene (tomato unigene accession SGN-U582631/Solyc09g082120.3.1) were generated and homozygous lines were obtained after four generations of self-pollination. In this study, <i>SlGlyI-</i>overepxressing line (GlyI), wild type (WT, negative control) and plants transforme  ...[more]

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