Proteomics

Dataset Information

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Target identification of SB2001 by thermal proteome profiling

ABSTRACT: In this study, we identified target proteins of SB2001, a cytotoxic agent that acts specifically against HeLa cells. Because SB2001 (= A08) lacked of chemical modification sites, label-free target identification methods using the thermal proteome profiling (TPP) were applied to characterize its mechanism of action. SB2024 (= A11) was used as a negative control compound.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Cervix Carcinoma

SUBMITTER: Hankum Park  

LAB HEAD: Seung Bum Park

PROVIDER: PXD009903 | Pride | 2019-11-04

REPOSITORIES: Pride

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Publications

Label-free target identification reveals oxidative DNA damage as the mechanism of a selective cytotoxic agent.

Park Hankum H   Park Seung Bum SB  

Chemical science 20190219 12

Phenotypic screening can not only identify promising first-in-class drug candidates, but can also reveal potential therapeutic targets or neomorphic functions of known proteins. In this study, we identified target proteins of SB2001, a cytotoxic agent that acts specifically against HeLa human cervical cancer cells. Because SB2001 lacks chemical modification sites, label-free target identification methods including thermal stability shift-based fluorescence difference in two-dimensional gel elect  ...[more]

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