Proteomics

Dataset Information

0

METTL13 modulates mRNA translation through methylation of eEF1A at Lys55 and the N-terminus, part 2


ABSTRACT: The experiment aimed at characterizing the methylation status of eEF1A in cells (HeLa) stressed using a panel of drugs including anisomycin, cycloheximide, 4NQO and adenosine dialdehyde (AdOx).

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (human)

DISEASE(S): Cervix Carcinoma

SUBMITTER: Magnus Jakobsson  

LAB HEAD: Jesper V Olsen

PROVIDER: PXD009914 | Pride | 2018-07-04

REPOSITORIES: Pride

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Publications


Eukaryotic elongation factor 1 alpha (eEF1A) delivers aminoacyl-tRNA to the ribosome and thereby plays a key role in protein synthesis. Human eEF1A is subject to extensive post-translational methylation, but several of the responsible enzymes remain unknown. Using a wide range of experimental approaches, we here show that human methyltransferase (MTase)-like protein 13 (METTL13) contains two distinct MTase domains targeting the N terminus and Lys55 of eEF1A, respectively. Our biochemical and str  ...[more]

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