Dataset Information

Characterization of the phospho-signalling mediated by the sodium-potassium ATPase in response to ouabain

ABSTRACT: In this project we applied a workflow for phosphoproteomics analysis to characterize the phosphorylation signalling that is triggered by the cardiotonic steroid ouabain. Ouabain is a ligand for the sodium-potassium ATPase (NKA). Low concentration of ouabain trigger oscillation of intracellular calcium concentration and modulate the phosphorylation of several signaling molecules, impacting cellular proliferation, apoptosis and cell-to-cell contacts. Monkey kidney cells (COS-7) were either treated for 10 or 20 minutes with 100 nM ouabain, or left untreated. We identify 15,348 phospho-sites, of which 1,941 are significantly regulated at either one of the time points employed. Analysis of the significantly regulated phospho-sites shed light on the molecular mechanisms by which ouabain affects cell junctions, proliferation and apoptosis.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Chlorocebus Sabaeus

TISSUE(S): Fibroblast, Kidney

SUBMITTER: Elena Panizza

LAB HEAD: Anita Aperia

PROVIDER: PXD010006 | Pride | 2019-09-13

REPOSITORIES: Pride

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Publications

Ouabain-regulated phosphoproteome reveals molecular mechanisms for Na+, K+-ATPase control of cell adhesion, proliferation, and survival.

Panizza Elena E Zhang Liang L Fontana Jacopo Maria JM Hamada Kozo K Svensson Daniel D Akkuratov Evgeny E EE Scott Lena L Mikoshiba Katsuhiko K Brismar Hjalmar H Lehtiö Janne J Aperia Anita A

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20190710 9

The ion pump Na+, K+-ATPase (NKA) is a receptor for the cardiotonic steroid ouabain. Subsaturating concentration of ouabain triggers intracellular calcium oscillations, stimulates cell proliferation and adhesion, and protects from apoptosis. However, it is controversial whether ouabain-bound NKA is considered a signal transducer. To address this question, we performed a global analysis of protein phosphorylation in COS-7 cells, identifying 2580 regulated phosphorylation eve ...[more]

PMID: 31199885

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Dataset Information

Characterization of the phospho-signalling mediated by the sodium-potassium ATPase in response to ouabain

Publications

Ouabain-regulated phosphoproteome reveals molecular mechanisms for Na<sup>+</sup>, K<sup>+</sup>-ATPase control of cell adhesion, proliferation, and survival.

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