Proteomics

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Identification of Missing Proteins in Human Olfactory Epithelial Tissue by Liquid Chromatography-Tandem Mass Spectrometry


ABSTRACT: We performed proteomic analyses of human olfactory epithelial tissue to identify missing proteins using liquid chromatography-tandem mass spectrometry. Using a next-generation proteomic pipeline with a <1.0% false discovery rate at the peptide and protein levels, we identified 3,731 proteins, among which five were missing proteins (P0C7M7, P46721, P59826, Q658L1, and Q8N434). We validated the identified missing proteins using the corresponding synthetic peptides. We also identified 49 and 50 alternative splicing variants mapped at the neXtProt and GENCODE databases, respectively, and 2,000 additional single amino acid variants.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Nasal Epithelium

SUBMITTER: Ji Eun Jeong  

LAB HEAD: Jong Shin Yoo

PROVIDER: PXD010025 | Pride | 2018-08-17

REPOSITORIES: Pride

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Publications

Identification of Missing Proteins in Human Olfactory Epithelial Tissue by Liquid Chromatography-Tandem Mass Spectrometry.

Hwang Heeyoun H   Jeong Ji Eun JE   Lee Hyun Kyoung HK   Yun Ki Na KN   An Hyun Joo HJ   Lee Bonghee B   Paik Young-Ki YK   Jeong Tae Seok TS   Yee Gi Taek GT   Kim Jin Young JY   Yoo Jong Shin JS  

Journal of proteome research 20180820 12


We performed proteomic analyses of human olfactory epithelial tissue to identify missing proteins using liquid chromatography-tandem mass spectrometry. Using a next-generation proteomic pipeline with a < 1.0% false discovery rate at the peptide and protein levels, we identified 3731 proteins, among which five were missing proteins (P0C7M7, P46721, P59826, Q658L1, and Q8N434). We validated the identified missing proteins using the corresponding synthetic peptides. No olfactory receptor (OR) prote  ...[more]

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