Dataset Information

Quantitative proteomics of potato leaves infected withPhytophthora infestans provides insights into coordinated and altered protein expression during early and late disease stages in Solanum tuberosum (cv. Sarpo Mira)

ABSTRACT: Potato (Solanum tuberosum) late blight disease caused by Phytophthora. infestans is one of the most critical crop diseases in the world. We investigated the proteome of Solanum tubersoum (cv. Sarpo Mira) by quantitative proteomics after foliar application of encysted zoospore suspension from P. infestans isolate. Also, we examined changes in protein abundance of cv. Sarpo Mira in response to P. infestans infection at three key timepoints 0 hpi (L), 48 hpi (M) and 120 hpi (H), divided into early and late disease stages and analyzed the protein-protein interaction during the disease stages. In response to P. infestans oomycete infection, the iTRAQ comparative proteome survey revealed a total of 1229 differentially expressed proteins (DEPs) in the pairwise comparison of disease stages, which included DEPs specific to early disease stage, DEPs specific to late disease stage and commonly shared DEPs. Over 80% of the change in protein abundance were up-regulated in the early stages of infection while about 61% of the DEPs in the later disease stage were down-regulated. The analysis of expression patterns, functional category, and enrichment tests highlighted significant coordination and enrichment of cell wall-associated defense response proteins in the early stages and cellular protein modification process as well as membrane protein complex formation in the late stages. Furthermore, strong protein interactions were observed in the late disease stage that might relate to disease pathogenesis.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Solanum Tuberosum (potato)

TISSUE(S): Leaf

SUBMITTER: qian zhou

LAB HEAD: Chunfang Xiao

PROVIDER: PXD010045 | Pride | 2019-01-09

REPOSITORIES: Pride

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Publications

Quantitative Proteomics of Potato Leaves Infected with Phytophthora infestans Provides Insights into Coordinated and Altered Protein Expression during Early and Late Disease Stages.

Xiao Chunfang C Gao Jianhua J Zhang Yuanxue Y Wang Zhen Z Zhang Denghong D Chen Qiaoling Q Ye Xingzhi X Xu Yi Y Yang Guocai G Yan Lei L Cheng Qun Q Chen Jiaji J Shen Yanfen Y

International journal of molecular sciences 20190101 1

In order to get a better understanding of protein association during Solanum tuberosum (cv. Sarpo Mira)⁻Phytophthora infestans incompatible interaction, we investigated the proteome dynamics of cv. Sarpo Mira, after foliar application of zoospore suspension from P. infestans isolate, at three key time-points: zero hours post inoculation (hpi) (Control), 48 hpi (EI), and 120 hpi (LI); divided into early and late disease stages by the tandem mass tagging (TMT) method. A total ...[more]

PMID: 30609684

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Dataset Information

Quantitative proteomics of potato leaves infected withPhytophthora infestans provides insights into coordinated and altered protein expression during early and late disease stages in Solanum tuberosum (cv. Sarpo Mira)

Publications

Quantitative Proteomics of Potato Leaves Infected with <i>Phytophthora infestans</i> Provides Insights into Coordinated and Altered Protein Expression during Early and Late Disease Stages.

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