Dataset Information

Mapping Proteome-Wide Targets of Protein Kinases in Response to Stresses

ABSTRACT: Protein kinases are major regulatory components in almost all cellular processes in eukaryotic cells. By adding phosphate groups, protein kinases regulate the activity, localization, protein-protein interactions and other profiles of their target proteins. It is known that protein kinases such as SNF1 related protein kinase 2 and 3 (SnRK2 and SnRK3), calcium dependent protein kinases (CDPKs), and mitogen activated protein kinases (MAPKs) are central components in plant response to environmental stresses such as drought, high salinity, cold and pathogen attack. Although the phosphorylation-dependent signaling plays critical roles in plant stress biology, only a few targets of these protein kinases have been identified. How these protein kinases regulate the downstream biological processes and mediate the stress responses are still largely unknown. In this study, we developed an isotope labeled protein kinase assay-based approach with enriched phosphoproteome as kinase-substrates pool to identify the putative substrates of 8 protein kinases that function in plant abiotic and biotic stress responses. As a result, we identified more than 5,081 putative targets of osmotic stress-activated SnRK2.4 and SnRK2.6, ABA activated protein kinases SnRK2.6 and Casein-Kinase Like 2 (CKL2), elicitor-activated protein kinase CDPK11 and MPK6, cold-activated protein kinase MPK6, H2O2-activated protein kinase OXI1 and MPK6, salt-induced protein kinase SOS1 and MPK6, as well as the low-potassium-activated protein kinase CIPK23. We also analyzed the conserved motif and functional ontology of these putative kinase targets. These results provide comprehensive information on the role of these protein kinases in the control of cellular activities and could be valuable resource for further study on the mechanism underlying plant response to environmental stresses.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Chuan-Chih Hsu

LAB HEAD: jian-Kang Zhu

PROVIDER: PXD010053 | Pride | 2020-01-16

REPOSITORIES: Pride

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Mapping proteome-wide targets of protein kinases in plant stress responses.

Wang Pengcheng P Hsu Chuan-Chih CC Du Yanyan Y Zhu Peipei P Zhao Chunzhao C Fu Xing X Zhang Chunguang C Paez Juan Sebastian JS Macho Alberto P AP Tao W Andy WA Zhu Jian-Kang JK

Proceedings of the National Academy of Sciences of the United States of America 20200128 6

Protein kinases are major regulatory components in almost all cellular processes in eukaryotic cells. By adding phosphate groups, protein kinases regulate the activity, localization, protein-protein interactions, and other features of their target proteins. It is known that protein kinases are central components in plant responses to environmental stresses such as drought, high salinity, cold, and pathogen attack. However, only a few targets of these protein kinases have been identified. Moreove ...[more]

PMID: 31992638

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Project description:Pathak2013 - MAPK activation in response to various abiotic stresses MAPK activation mechanism in response to various abiotic stress conditions, such as cold, salt, drought, H2O2, heavy metal and ethylene, in plants This model is described in the article: Modeling of the MAPK machinery activation in response to various abiotic and biotic stresses in plants by a system biology approach. Pathak RK, Taj G, Pandey D, Arora S, Kumar A. Bioinformation 2013; 9(9): 443-449 Abstract: Mitogen-Activated Protein Kinases (MAPKs) cascade plays an important role in regulating plant growth and development, generating cellular responses to the extracellular stimuli. MAPKs cascade mainly consist of three sub-families i.e. mitogen-activated protein kinase kinase kinase (MAPKKK), mitogen-activated protein kinase kinase (MAPKK) and mitogen activated protein kinase (MAPK), several cascades of which are activated by various abiotic and biotic stresses. In this work we have modeled the holistic molecular mechanisms essential to MAPKs activation in response to several abiotic and biotic stresses through a system biology approach and performed its simulation studies. As extent of abiotic and biotic stresses goes on increasing, the process of cell division, cell growth and cell differentiation slow down in time dependent manner. The models developed depict the combinatorial and multicomponent signaling triggered in response to several abiotic and biotic factors. These models can be used to predict behavior of cells in event of various stresses depending on their time and exposure through activation of complex signaling cascades. This model is hosted on BioModels Database and identified by: BIOMD0000000491 . To cite BioModels Database, please use: BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models . To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information.

Dataset Information

Mapping Proteome-Wide Targets of Protein Kinases in Response to Stresses

Publications

Mapping proteome-wide targets of protein kinases in plant stress responses.

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