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Proteome-wide analysis of cysteine reactivity in Arabidopsis associated with biotic stress response

ABSTRACT: We used a method for the enrichment of proteins with oxidative post-translational modifications that enabled the comprehensive identification and label-free quantification of reversibly oxidized thiols across the plant proteome. The redoxomes were characterized in wild-type and the top1top2 null mutants in the early stages of effector-triggered immunity.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Leaf

SUBMITTER: Evan McConnell

LAB HEAD: Leslie M. Hicks

PROVIDER: PXD010104 | Pride | 2019-03-19

REPOSITORIES: Pride

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Proteome-Wide Analysis of Cysteine Reactivity during Effector-Triggered Immunity.

McConnell Evan W EW Berg Philip P Westlake Timothy J TJ Wilson Katherine M KM Popescu George V GV Hicks Leslie M LM Popescu Sorina C SC

Plant physiology 20181203 4

A surge in the accumulation of oxidants generates shifts in the cellular redox potential during early stages of plant infection with pathogens and activation of effector-triggered immunity (ETI). The redoxome, defined as the proteome-wide oxidative modifications of proteins caused by oxidants, has a well-known impact on stress responses in metazoans. However, the identity of proteins and the residues sensitive to oxidation during the plant immune response remain largely unknown. Previous studies ...[more]

PMID: 30510037

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