Proteomics

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Tomato another proteome - Response mechanisms induced by exposure to high temperature in anthers from thermo-tolerant and thermo-sensitive tomato plants: A proteomic perspective


ABSTRACT: The study was aimed to investigate by differential proteomics heat stress response mechanisms in tomato anthers collected from flowers of Solanum lycopersicum cv Saladette (SAL), already annotated as a thermo-tolerant tomato genotype and flowers from Solanum lycopersicum cv M82, reported as a thermo-sensitive genotype. Proteins constitutively present at higher or lower level in the tolerant genotype and/or proteins whose abundance was modulated by growth under high temperature in both genotypes were identified, thus leading to define processes involved in the heat stress response and responsible for efficient reduction of the adverse effects of HS. Results showed differences in the abundance of ninety-six proteins and their functional classification highlighted that heat stress mainly affected metabolic pathways, such as energy metabolism (glycolysis and sucrose degradation), nitrogen assimilation and amino acid biosynthesis and modulated the expression of proteins involved in the folding and degradation machinery and ROS detoxification systems

INSTRUMENT(S): Waters instrument model

ORGANISM(S): Solanum Lycopersicum

TISSUE(S): Anther

SUBMITTER: Maria Fiorella Mazzeo  

LAB HEAD: Maria Fiorella Mazzeo

PROVIDER: PXD010156 | Pride | 2018-07-25

REPOSITORIES: Pride

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Response mechanisms induced by exposure to high temperature in anthers from thermo-tolerant and thermo-sensitive tomato plants: A proteomic perspective.

Mazzeo Maria Fiorella MF   Cacace Giuseppina G   Iovieno Paolo P   Massarelli Immacolata I   Grillo Stefania S   Siciliano Rosa Anna RA  

PloS one 20180719 7


Constant global warming is one of the most detrimental environmental factors for agriculture causing significant losses in productivity as heat stress (HS) conditions damage plant growth and reproduction. In flowering plants such as tomato, HS has drastic repercussions on development and functionality of male reproductive organs and pollen. Response mechanisms to HS in tomato anthers and pollen have been widely investigated by transcriptomics; on the contrary, exhaustive proteomic evidences are  ...[more]

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