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Glioblastoma cells´ response to a cancer drug

ABSTRACT: An untargeted proteomics analysis was undertaken to investigate responses to a cancer drug (survivin inhibitor) and look for possible prognostic biomarkers. A non-responcive cell line(T1454) and a sensitive cell line (T1459) (according to YM155 EC50 values) were selected (controls and treated cells). The same samples were anaysed using NMR (metabolomics).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

DISEASE(S): Brain Glioblastoma Multiforme

SUBMITTER: Henriette Engen Berg

LAB HEAD: Henriette Engen Berg

PROVIDER: PXD010180 | Pride | 2019-04-10

REPOSITORIES: Pride

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Nuclear Magnetic Resonance Spectroscopy to Identify Metabolite Biomarkers of Nonresponsiveness to Targeted Therapy in Glioblastoma Tumor Stem Cells.

Hvinden Ingvild Comfort IC Berg Henriette Engen HE Sachse Daniel D Skaga Erlend E Skottvoll Frøydis Sved FS Lundanes Elsa E Sandberg Cecilie J CJ Vik-Mo Einar O EO Rise Frode F Wilson Steven Ray SR

Journal of proteome research 20190418 5

Glioblastoma is the most common and malignant brain tumor, and current therapies confer only modest survival benefits. A major obstacle is our ability to monitor treatment effect on tumors. Current imaging modalities are ambiguous, and repeated biopsies are not encouraged. To scout for markers of treatment response, we used NMR spectroscopy to study the effects of a survivin inhibitor on the metabolome of primary glioblastoma cancer stem cells. Applying high resolution NMR spectroscopy (<sup>1</ ...[more]

PMID: 30964684

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