Dataset Information

Loqs2 (AAEL013721) interactome in Aedes aegypti Aag2 cells (FLAG-tag)

ABSTRACT: Loquacious-2 (Loqs2) is a recently identified paralog of Loquacious found in Aedes aegypti. Data presented here allowed the proteomic characterization of Loqs2 interactome in Aedes aegypti Aag2 cells. We carried out immunoprecipitation experiments where Loqs2 tagged protein was precipitated along with its partners. Immunoprecipitation of tagged eGFP protein was performed in the same conditions to verify the specificity of these associations. Experiments were performed in biological triplicates.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Aedes Aegypti

SUBMITTER: Lauriane Kuhn

LAB HEAD: Elisa Andrade

PROVIDER: PXD010191 | Pride | 2018-11-21

REPOSITORIES: Pride

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Publications

Control of dengue virus in the midgut of Aedes aegypti by ectopic expression of the dsRNA-binding protein Loqs2.

Olmo Roenick P RP Ferreira Alvaro G A AGA Izidoro-Toledo Tatiane C TC Aguiar Eric R G R ERGR de Faria Isaque J S IJS de Souza Kátia P R KPR Osório Kátia P KP Kuhn Lauriane L Hammann Philippe P de Andrade Elisa G EG Todjro Yaovi Mathias YM Rocha Marcele N MN Leite Thiago H J F THJF Amadou Siad C G SCG Armache Juliana N JN Paro Simona S de Oliveira Caroline D CD Carvalho Fabiano D FD Moreira Luciano A LA Marois Eric E Imler Jean-Luc JL Marques João T JT

Nature microbiology 20181029 12

Dengue virus (DENV) is an arbovirus transmitted to humans by Aedes mosquitoes<sup>1</sup>. In the insect vector, the small interfering RNA (siRNA) pathway is an important antiviral mechanism against DENV<sup>2-5</sup>. However, it remains unclear when and where the siRNA pathway acts during the virus cycle. Here, we show that the siRNA pathway fails to efficiently silence DENV in the midgut of Aedes aegypti although it is essential to restrict systemic replication. Accumulation of DENV-derived s ...[more]

PMID: 30374169

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Project description:Female Aedes aegypti mosquitoes impose a severe global public health burden as primary vectors of multiple viral and parasitic pathogens. Under optimal environmental conditions, Aedes aegypti females have access to human hosts that provide blood proteins for egg development, conspecific males that provide sperm for fertilization, and freshwater that serves as an egg-laying substrate suitable for offspring survival. As global temperatures rise, Aedes aegypti females are faced with climate challenges, like intense droughts and intermittent precipitation, which create unpredictable and suboptimal conditions for the egg-laying step of their reproductive cycle. Aedes aegypti mosquitoes nonetheless show remarkable reproductive resilience, but how they achieve this is unknown. Here we show that under drought-like conditions simulated in the laboratory, mated, blood-fed Aedes aegypti females carrying mature eggs retain them in their ovaries for extended periods, while maintaining the viability of these eggs until they can be deposited in freshwater. Using transcriptomic and proteomic profiling of Aedes aegypti ovaries, we identify two previously uncharacterized genes – here named tweedledee and tweedledum – that show ovary-enriched, temporally-restricted expression during egg retention. These genes are mosquito-specific, linked within a syntenic locus, and rapidly evolving under positive selection, raising the possibility that they serve an adaptive function. Using loss-of-function mutagenesis to disrupt both genes, we show that, tweedledee and tweedledum, which encode secreted proteins, are specifically required for extended retention of viable eggs, such as during intermittent precipitation or drought. These results highlight an elegant example of taxon-restricted genes at the heart of an important adaptation that equips Aedes aegypti females with “insurance” to, when contextually appropriate, flexibly extend their reproductive sequence without losing reproductive capacity, thus allowing this species to exploit diverse and unpredictable habitats.

Dataset Information

Loqs2 (AAEL013721) interactome in Aedes aegypti Aag2 cells (FLAG-tag)

Publications

Control of dengue virus in the midgut of Aedes aegypti by ectopic expression of the dsRNA-binding protein Loqs2.

Similar Datasets

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