Name: ATR-mediated Proteome Remodeling is a Major Determinant of Homologous Recombination Capacity in Cancer Cells
Keywords: Atr inhibitor

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ATR-mediated Proteome Remodeling is a Major Determinant of Homologous Recombination Capacity in Cancer Cells

ABSTRACT: DMSO or 5uM ATR inhibitor(VE-821) were treated for 8 days on U2OS cells prior to anlaysis.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Osteoblast

DISEASE(S): Bone Osteosarcoma

SUBMITTER: Dongsung Kim

LAB HEAD: Marcus Smolka

PROVIDER: PXD010223 | Pride | 2018-07-17

REPOSITORIES: Pride

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ATR-mediated proteome remodeling is a major determinant of homologous recombination capacity in cancer cells.

Kim Dongsung D Liu Yi Y Oberly Susannah S Freire Raimundo R Smolka Marcus B MB

Nucleic acids research 20180901 16

The ATR kinase is crucial for genome maintenance, but the mechanisms by which ATR controls the DNA repair machinery are not fully understood. Here, we find that long-term chronic inhibition of ATR signaling severely impairs the ability of cells to utilize homologous recombination (HR)-mediated DNA repair. Proteomic analysis shows that chronic ATR inhibition depletes the abundance of key HR factors, suggesting that spontaneous ATR signaling enhances the capacity of cells to use HR-mediated repair ...[more]

PMID: 30010936

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