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Identification of potential biomarkers in testicular cancer by SILAC and mass spectrometry

ABSTRACT: This study represents a proteomic resource for testis cancer with quantitative protein expression data for 5236 proteins. By quantitative mass-spectrometry-based proteomics, we compared the two Malignant germ cell tumour cell lines, TCam-2 with seminoma-like characteristics, and NTERA-2, an embryonal carcinoma-like cell line.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Hanibal Bohnenberger

LAB HEAD: Thomas Oellerich

PROVIDER: PXD010275 | Pride | 2019-11-12

REPOSITORIES: Pride

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Proteomic Comparison of Malignant Human Germ Cell Tumor Cell Lines.

Bremmer Felix F Bohnenberger Hanibal H Küffer Stefan S Oellerich Thomas T Serve Hubert H Urlaub Henning H Strauss Arne A Maatoug Yasmine Y Behnes Carl Ludwig CL Oing Christoph C Radzun Heinz Joachim HJ Ströbel Philipp P Balabanov Stefan S Honecker Friedemann F

Disease markers 20190903

Malignant germ cell tumors (GCT) are the most common malignant tumors in young men between 18 and 40 years. The correct identification of histological subtypes, in difficult cases supported by immunohistochemistry, is essential for therapeutic management. Furthermore, biomarkers may help to understand pathophysiological processes in these tumor types. Two GCT cell lines, TCam-2 with seminoma-like characteristics, and NTERA-2, an embryonal carcinoma-like cell line, were compared by a quantitative ...[more]

PMID: 31565104

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