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Short enzymatic reactions in proteomics studies

ABSTRACT: SKBR3 breast cancer cell extracts were digested with trypsin (or LysC) on short time scales (7min, 15min, 30min, 1h, and 18h), and the results were compared to overnight digestion protocols.

INSTRUMENT(S): LTQ

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Iulia M. Lazar

LAB HEAD: Iulia Lazar

PROVIDER: PXD010306 | Pride | 2018-09-24

REPOSITORIES: Pride

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Partial enzymatic reactions: A missed opportunity in proteomics research.

Deng Jingren J Julian Morgan H MH Lazar Iulia M IM

Rapid communications in mass spectrometry : RCM 20181201 23

<h4>Rationale</h4>Biological studies are conducted at ever-increasing rates by relying on proteomic workflows. Although data acquisition by mass spectrometry is highly automated and rapid, sample preparation continues to be the bottleneck of developing high-throughput workflows. Enzymatic protein processing, in particular, involves time-consuming protocols that can extend from one day to another. To address this gap, we developed and evaluated simple, in-solution tryptic enzymatic reactions that ...[more]

PMID: 30221418

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