Dataset Information
Biological Plasticity Rescues Target Activity in CRISPR Knock Outs
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion Lumos, Q Exactive HF-X
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Hap-1 Cell, K-562 Cell, Cell Line Cell
SUBMITTER: 
Maria Faelth Savitski
LAB HEAD: Marcus Bantscheff
PROVIDER: PXD010335 | Pride | 2019-09-03
REPOSITORIES: Pride
Publications
Biological plasticity rescues target activity in CRISPR knock outs.
Nature methods 20191028 11
Gene knock outs (KOs) are efficiently engineered through CRISPR-Cas9-induced frameshift mutations. While the efficiency of DNA editing is readily verified by DNA sequencing, a systematic understanding of the efficiency of protein elimination has been lacking. Here we devised an experimental strategy combining RNA sequencing and triple-stage mass spectrometry to characterize 193 genetically verified deletions targeting 136 distinct genes generated by CRISPR-induced frameshifts in HAP1 cells. We o ...[more]
