Optimising size exclusion chromatography for extracellular vesicle enrichment and proteomic analysis from clinically relevant samples

ABSTRACT: We performed a quantitative evaluation and optimization of SEC for separating EVs from contaminating proteins. This optimized method was applied to enrich EVs from healthy plasma and MDA-MB-231 cancer cell culture medium. By spiking-in cancer cell-derived EVs to healthy plasma and then performing SEC enrichment, we were subsequently to detect cancer cell line- specific proteins by LC-MS/MS. We quantified the limit of detection and showed that cancer EV-associated proteins were detectable by nano-LC-MS/MS when as little as 1% of the total plasma EV number were derived from a cancer cell line.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma, Epithelial Cell, Cell Culture

SUBMITTER: Rebecca Lane  

LAB HEAD: Michelle M Hill

PROVIDER: PXD010341 | Pride | 2019-01-16

REPOSITORIES: Pride