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CPTAC High-grade serous ovarian carcinoma SWATH-MS

ABSTRACT: Sequential Window Acquisition of All Theoretical Mass Spectra (SWATH-MS) is a DIA method whose use in proteomic studies has increased considerably within the past five years. SWATH-MS acquires a complete and permanent digital record for all the detectable MS/MS spectra of a sample using DIA. After their generation, the SWATH-MS maps can be used for iterative analyses of candidate proteins. As with any analytical methodology that has potential widespread use, several studies have been conducted to optimize and evaluate the performance of SWATH-MS. The present dataset was acquired from 103 tissue samples of high-grade serous ovarian carcinoma collected and processed in the context of the CPTAC initiative and analyzed via bottom-up SWATH mass spectrometry.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Ovary, Ovarian Surface Epithelial Cell

DISEASE(S): Malignant Neoplasm Of Ovary

SUBMITTER: Betty Friedrich

LAB HEAD: Rudolf Aebersold

PROVIDER: PXD010437 | Pride | 2020-07-14

REPOSITORIES: Pride

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Publications

Orthogonal Proteomic Platforms and Their Implications for the Stable Classification of High-Grade Serous Ovarian Cancer Subtypes.

Thomas Stefani N SN Friedrich Betty B Schnaubelt Michael M Chan Daniel W DW Zhang Hui H Aebersold Ruedi R

iScience 20200418 6

The National Cancer Institute (NCI) Clinical Proteomic Tumor Analysis Consortium (CPTAC) established a harmonized method for large-scale clinical proteomic studies. SWATH-MS, an instance of data-independent acquisition (DIA) proteomic methods, is an alternate proteomic approach. In this study, we used SWATH-MS to analyze remnant peptides from the original retrospective TCGA samples generated for the CPTAC ovarian cancer proteogenomic study. The SWATH-MS results recapitulated the confident identi ...[more]

PMID: 32534439

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