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Analysis of UCHL1 knockout in brain

ABSTRACT: We analyzed the brain of 5 day old mice with homozygous and heterozygous brain specific UCHL1 knockout using nLC-MS/MS.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Markus Rinschen

LAB HEAD: Markus Rinschen

PROVIDER: PXD010449 | Pride | 2019-04-02

REPOSITORIES: Pride

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Ubiquitin C-terminal hydrolase L1 (UCH-L1) loss causes neurodegeneration by altering protein turnover in the first postnatal weeks.

Reinicke Anna T AT Laban Karoline K Sachs Marlies M Kraus Vanessa V Walden Michael M Damme Markus M Sachs Wiebke W Reichelt Julia J Schweizer Michaela M Janiesch Philipp Christoph PC Duncan Kent E KE Saftig Paul P Rinschen Markus M MM Morellini Fabio F Meyer-Schwesinger Catherine C

Proceedings of the National Academy of Sciences of the United States of America 20190328 16

Ubiquitin C-terminal hydrolase L1 (UCH-L1) is one of the most abundant and enigmatic enzymes of the CNS. Based on existing UCH-L1 knockout models, UCH-L1 is thought to be required for the maintenance of axonal integrity, but not for neuronal development despite its high expression in neurons. Several lines of evidence suggest a role for UCH-L1 in mUB homeostasis, although the specific in vivo substrate remains elusive. Since the precise mechanisms underlying UCH-L1-deficient neurodegeneration re ...[more]

PMID: 30923110

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