Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap Velos
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Brain
SUBMITTER: Lucas Baltussen
LAB HEAD: Sila Ultanir
PROVIDER: PXD010511 | Pride | 2018-09-18
REPOSITORIES: Pride
Baltussen Lucas L LL Negraes Priscilla D PD Silvestre Margaux M Claxton Suzanne S Moeskops Max M Christodoulou Evangelos E Flynn Helen R HR Snijders Ambrosius P AP Muotri Alysson R AR Ultanir Sila K SK
The EMBO journal 20180928 24
Loss-of-function mutations in CDKL5 kinase cause severe neurodevelopmental delay and early-onset seizures. Identification of CDKL5 substrates is key to understanding its function. Using chemical genetics, we found that CDKL5 phosphorylates three microtubule-associated proteins: MAP1S, EB2 and ARHGEF2, and determined the phosphorylation sites. Substrate phosphorylations are greatly reduced in CDKL5 knockout mice, verifying these as physiological substrates. In CDKL5 knockout mouse neurons, dendri ...[more]