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Project title: 20170712-Leslie-1

ABSTRACT: 190 proteins were identified from three samples. Among them41 proteins were detected in sample Layer 1 and/or Layer 2 but not in sample Layer 3, and one protein (SPCS2) was detected all three samples but its relative abundance is higher in Layer 1 than that in Layer 3. These 42 proteins can be considered as the most possible candidates proteins which are specifically present in the secretory granules prepared in this experiment.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Salivary Gland

SUBMITTER: Xianglin Zhai

LAB HEAD: Alex Zhai

PROVIDER: PXD010548 | Pride | 2018-10-16

REPOSITORIES: Pride

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Publications

A molecular switch orchestrates enzyme specificity and secretory granule morphology.

Ji Suena S Samara Nadine L NL Revoredo Leslie L Zhang Liping L Tran Duy T DT Muirhead Kayla K Tabak Lawrence A LA Ten Hagen Kelly G KG

Nature communications 20180829 1

Regulated secretion is an essential process where molecules destined for export are directed to membranous secretory granules, where they undergo packaging and maturation. Here, we identify a gene (pgant9) that influences the structure and shape of secretory granules within the Drosophila salivary gland. Loss of pgant9, which encodes an O-glycosyltransferase, results in secretory granules with an irregular, shard-like morphology, and altered glycosylation of cargo. Interestingly, pgant9 undergoe ...[more]

PMID: 30158631

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20170712-Leslie-3 - A molecular switch orchestrates enzyme specificity and secretory granule morphology

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