Dataset Information

293FT Cell Endogenous FANCA Protein-Protein Interactions

ABSTRACT: Hyperinsulinemia affects 72% of Fanconi anemia (FA) patients and an additional 25% experience lowered glucose tolerance or frank diabetes. The underlying molecular mechanisms contributing to the dysfunction of FA pancreas β cells is unknown. These experiments were performed in 293FT HEK cells as a part of the optimization and validation of the endogenous IP and to establish the FANCA interactome in a non-pancreas cell line to delineate beta cell-specific FANCA interactions.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Early Embryonic Cell

DISEASE(S): Fanconi Anemia Complementation Group A

SUBMITTER: Nicholas Woods

LAB HEAD: Nicholas T. Woods, Ph.D.

PROVIDER: PXD010570 | Pride | 2019-07-25

REPOSITORIES: Pride

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Publications

Delineating the role of FANCA in glucose-stimulated insulin secretion in β cells through its protein interactome.

Lagundžin Dragana D Hu Wen-Feng WF Law Henry C H HCH Krieger Kimiko L KL Qiao Fangfang F Clement Emalie J EJ Drincic Andjela T AT Nedić Olgica O Naldrett Michael J MJ Alvarez Sophie S Woods Nicholas T NT

PloS one 20190828 8

Hyperinsulinemia affects 72% of Fanconi anemia (FA) patients and an additional 25% experience lowered glucose tolerance or frank diabetes. The underlying molecular mechanisms contributing to the dysfunction of FA pancreas β cells is unknown. Therefore, we sought to evaluate the functional role of FANCA, the most commonly mutated gene in FA, in glucose-stimulated insulin secretion (GSIS). This study reveals that FANCA or FANCB knockdown impairs GSIS in human pancreas β cell line EndoC-βH3. To ide ...[more]

PMID: 31461451

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Project description:The sea-run phenotype of rainbow trout (Oncorhynchus mykiss), like other anadromous salmonids, present a juvenile stage fully adapted to life in freshwater known as parr. Development in freshwater is followed by the smolt stage, where preadaptations needed for seawater life are developed making fish ready to migrate to the ocean, after which event they become post-smolts. While these three life stages have been studied using a variety of approaches, proteomics has never been used for such purpose. The present study characterised the blood plasma proteome of parr, smolt and post-smolt rainbow trout using a gel electrophoresis liquid chromatography tandem mass spectrometry approach alone or in combination with low-abundant protein enrichment technology (combinatorial peptide ligand library). In total, 1,822 proteins were quantified, 17.95% of them being detected only in plasma post enrichment. Across all life stages, the most abundant proteins were ankyrin-2, DNA primase large subunit, actin, serum albumin, apolipoproteins, hemoglobin subunits, hemopexin-like proteins and complement C3. When comparing the different life stages, 17 proteins involved in mechanisms to cope with hyperosmotic stress and retinal changes, as well as the downregulation of nonessential processes in smolts, were significantly different between parr and smolt samples. On the other hand, 11 proteins related to increased growth in post-smolts, and also related to coping with hyperosmotic stress and to retinal changes, were significantly different between smolt and post-smolt samples. Overall, this study presents a series of proteins with the potential to complement current seawater-readiness assessment tests in rainbow trout, which can be measured non-lethally in an easily accessible biofluid. Furthermore, this study represents a first in-depth characterisation of the rainbow trout blood plasma proteome, having considered three life stages of the fish and used both fractionation alone or in combination with enrichment methods to increase protein detection.

Dataset Information

293FT Cell Endogenous FANCA Protein-Protein Interactions

Publications

Delineating the role of FANCA in glucose-stimulated insulin secretion in β cells through its protein interactome.

Similar Datasets

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