A combined N-terminomics and shotgun proteomics approach to investigate the responses of human cells to rapamycin and zinc at the mitochondrial level

ABSTRACT: This study aims investigating the impact of rapamycin and zinc at subtoxic doses on the human mitochondrial proteome. Experiments were performed on U397 monocytic cells. A dN-TOP strategy, combining shotgun proteomics and N-terminomics, was applied to study both internal and N-terminal peptides of the mitochondrial proteins.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Monocyte, Cell Culture

SUBMITTER: Joanna Bons  

LAB HEAD: Christine Carapito

PROVIDER: PXD010572 | Pride | 2019-05-14

REPOSITORIES: Pride