Proteomics

Dataset Information

0

Multi-Omic Profiling Reveals Dynamics of the Phased Progression of Pluripotency


ABSTRACT: Pluripotency is highly dynamic and progresses through a continuum of pluripotent stem-cell states. The two states that bookend the pluripotency continuum, naïve and primed, are well characterized, but our understanding of the intermediate states and transitions between them remain incomplete. Here, we dissect the dynamics of pluripotent state transitions underlying pre- to post-implantation epiblast differentiation. Through comprehensive mapping of the proteome, phosphoproteome, transcriptome, and epigenome of embryonic stem cells transitioning from naïve to primed pluripotency, we find that rapid, acute, and widespread changes to the phosphoproteome precede ordered changes to the epigenome, transcriptome, and proteome. Reconstruction of kinase-substrate networks reveals signaling cascades, dynamics, and crosstalk. Distinct waves of global proteomic changes mark discrete phases of pluripotency, with cell state-specific surface markers tracking pluripotent state transitions. Our data provide new insights into the multi-layered control of the phased progression of pluripotency and a foundation for modeling mechanisms regulating pluripotent state transitions (www.stemcellatlasorg).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus musculus  

TISSUE(S): Embryo

DISEASE(S): Not Available

SUBMITTER: Mario Oroshi  

LAB HEAD: Matthias Mann

PROVIDER: PXD010621 | Pride | 2019-05-07

REPOSITORIES: pride

altmetric image

Publications


Pluripotency is highly dynamic and progresses through a continuum of pluripotent stem cell states. The two states that bookend the pluripotency continuum, naive and primed, are well characterized, but our understanding of the intermediate states and transitions between them remains incomplete. Here, we dissect the dynamics of pluripotent state transitions underlying pre- to post-implantation epiblast differentiation. Through comprehensive mapping of the proteome, phosphoproteome, transcriptome,  ...[more]

Similar Datasets

| GSE61994 | GEO
| GSE99488 | GEO
2019-07-04 | PXD011782 | Pride
2014-06-05 | E-GEOD-56096 | ArrayExpress
| GSE105054 | GEO
2014-06-05 | E-GEOD-56098 | ArrayExpress
2013-01-18 | E-GEOD-42580 | ArrayExpress
2018-09-24 | GSE119378 | GEO
2015-09-14 | E-GEOD-70545 | ArrayExpress
| GSE99491 | GEO