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Podocyte Focal Adhesion Proteome

ABSTRACT: Expression proteomics analysis of focal adhesion complexes isolated from a human immortalized podocyte cell line.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Podocyte (sensu Diptera), Kidney

SUBMITTER: Joern Dengjel

LAB HEAD: Jörn Dengjel

PROVIDER: PXD010626 | Pride | 2018-12-14

REPOSITORIES: Pride

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ARP3 Controls the Podocyte Architecture at the Kidney Filtration Barrier.

Schell Christoph C Sabass Benedikt B Helmstaedter Martin M Geist Felix F Abed Ahmed A Yasuda-Yamahara Mako M Sigle August A Maier Jasmin I JI Grahammer Florian F Siegerist Florian F Artelt Nadine N Endlich Nicole N Kerjaschki Dontscho D Arnold Hans-Henning HH Dengjel Jörn J Rogg Manuel M Huber Tobias B TB

Developmental cell 20181129 6

Podocytes, highly specialized epithelial cells, build the outer part of the kidney filtration barrier and withstand high mechanical forces through a complex network of cellular protrusions. Here, we show that Arp2/3-dependent actin polymerization controls actomyosin contractility and focal adhesion maturation of podocyte protrusions and thereby regulates formation, maintenance, and capacity to adapt to mechanical requirements of the filtration barrier. We find that N-WASP-Arp2/3 define the devel ...[more]

PMID: 30503751

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