Dataset Information

Anoxygenic Photosynthesis and Dark Carbon Metabolism in the Purple Sulfur Bacterium “Thiodictyon syntrophicum“ nov. strain Cad16T

ABSTRACT: Strain Cad16T was previously shown to fix a major fraction of the bulk CO2 under both light and dark conditions and was suggested to be involved in Fe(II) driven aerobic metabolism in the lake Cadagno chemocline. However evidence on genomic and proteomic level was only fragmentary . In order to understand the biological functions expressed under phototrophic and chemotrophic growth conditions of str. Cad16T an in situ experiment conceived. It included cultures of str. Cad16T incubated in dialysis bags for three months while physiochemical conditions were monitored longitudinally. Upon sampling, physiological differences of str. Cad16T were compared utilizing quantitative proteomics in combination with CO2-uptake measurements.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Candidatus Thiodictyon Syntrophicum Str. Cad16

TISSUE(S): Cell Suspension Culture

SUBMITTER: Samuel Luedin

LAB HEAD: Mauro Tonolla

PROVIDER: PXD010641 | Pride | 2019-03-29

REPOSITORIES: Pride

ACCESS DATA

Publications

Mixotrophic Growth Under Micro-Oxic Conditions in the Purple Sulfur Bacterium "Thiodictyon syntrophicum".

Luedin Samuel M SM Storelli Nicola N Danza Francesco F Roman Samuele S Wittwer Matthias M Pothier Joël F JF Tonolla Mauro M

Frontiers in microbiology 20190305

The microbial ecosystem of the meromictic Lake Cadagno (Ticino, Swiss Alps) has been studied intensively in order to understand structure and functioning of the anoxygenic phototrophic sulfur bacteria community living in the chemocline. It has been found that the purple sulfur bacterium "Thiodictyon syntrophicum" strain Cad16T, belonging to the Chromatiaceae, fixes around 26% of all bulk inorganic carbon in the chemocline, both during day and night. With this study, we elucidat ...[more]

PMID: 30891015

Similar Datasets

Project description:Paget disease of bone (PDB) is a chronic skeletal disorder with contemporary cases characterised by one or a few affected bones in individuals over 55 years of age. PDB-like changes have been noted in archaeological remains as old as Roman although accurate diagnoses and knowledge of the natural history of ancient forms of the disease are lacking. Previous macroscopic and radiographic analyses of six skeletons from a collection of 130 excavated at Norton Priory in Cheshire, UK, and dating to late Medieval times, noted unusually extensive pathological changes resembling PDB affecting up to 75% of individual skeletons. Here we report the prevalence of the disease in the collection is also remarkably high (at least 15.8% of the adult sample) with age-at-death estimations as low as 35 years. Despite these profound phenotypic differences paleoproteomic analyses identified SQSTM1/p62 (p62), a protein central to the pathological milieu of classical PDB, as one of the few non-collagenous human sequences preserved in skeletal samples, indicating that the disorder was likely an ancient precursor of contemporary PDB. Western blotting indicated abnormal migration of ancient p62 protein, with subsequent targeted proteomic analyses detecting more than 60% of the p62 primary sequence and directing sequencing analyses of ancient DNA that excluded contemporary PDB-associated SQSTM1 mutations. Together our observations indicate the ancient p62 protein is likely modified within its C-terminal ubiquitin-associated (UBA) domain. Ancient miRNAs were also remarkably well preserved in an osteosarcoma from a skeleton with extensive disease, with miR-16 expression changes consistent with that reported in contemporary PDB-associated bone tumours. Our work demonstrates the potential of proteomics to inform diagnoses of ancient disease and supports the proposal that Medieval Norton Priory was a ‘hotspot’ for an ancient form of PDB, with unusual features presumably potentiated by as yet unidentified environmental or genetic factors.

Dataset Information

Anoxygenic Photosynthesis and Dark Carbon Metabolism in the Purple Sulfur Bacterium “Thiodictyon syntrophicum“ nov. strain Cad16T

Publications

Mixotrophic Growth Under Micro-Oxic Conditions in the Purple Sulfur Bacterium "<i>Thiodictyon syntrophicum</i>".

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure