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Molecular profiling of innate immune response mechanisms in ventilator associated pneumonia (VAP)

ABSTRACT: Ventilator associated pneumonia (VAP) is the 2nd most common hospital acquired infection associated with high morbidity, mortality and increased hospitalization. Current practice of diagnosis is based on clinical symptoms and bronchoalvolar lavage (BAL) culture. The procedures of BAL collections are invasive whereas, endotracheal aspirate (ETA), a matrix of upper airway collection is minimally invasive and underexplored in VAP diagnosis. The study describes first in detail characterization of proteome of longitudinal ETA collections from 16 intubated patients including 11 VAP patients and explores potential utility of ETA in VAP diagnosis.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Lung, Bronchoalveolar Lavage

DISEASE(S): Pneumonia

SUBMITTER: Krystine Mansfield

LAB HEAD: Patrick Pirrotte

PROVIDER: PXD010715 | Pride | 2020-07-27

REPOSITORIES: Pride

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Molecular Profiling of Innate Immune Response Mechanisms in Ventilator-associated Pneumonia.

Pathak Khyatiben V KV McGilvrey Marissa I MI Hu Charles K CK Garcia-Mansfield Krystine K Lewandoski Karen K Eftekhari Zahra Z Yuan Yate-Ching YC Zenhausern Frederic F Menashi Emmanuel E Pirrotte Patrick P

Molecular & cellular proteomics : MCP 20200724 10

Ventilator-associated pneumonia (VAP) is a common hospital-acquired infection, leading to high morbidity and mortality. Currently, bronchoalveolar lavage (BAL) is used in hospitals for VAP diagnosis and guiding treatment options. Although BAL collection procedures are invasive, alternatives such as endotracheal aspirates (ETA) may be of diagnostic value, however, their use has not been thoroughly explored. Longitudinal ETA and BAL were collected from 16 intubated patients up to 15 days, of which ...[more]

PMID: 32709677

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