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Proteomics of cerebrospinal fluid (CSF) of acitretin treated patients

ABSTRACT: Acitretin has been shown to increase ADAM10 expression. In a phase II clinical trial, Alzheimer's disease patients were treated with acitretin or placebo to increase ADAM10 levels to counteract amyloid beta production. Analysis of the cerebrospinal fluid of from 18 AD patients treated with the synthetic retinoid acitretin or placebo were analyzed by label-free quantitative LC-MS/MS analysis.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cerebrospinal Fluid

DISEASE(S): Alzheimer's Disease

SUBMITTER: Stephan Mueller

LAB HEAD: Stefan F. Lichtenthaler

PROVIDER: PXD010756 | Pride | 2019-04-06

REPOSITORIES: Pride

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NrCAM is a marker for substrate-selective activation of ADAM10 in Alzheimer's disease.

Brummer Tobias T Müller Stephan A SA Pan-Montojo Francisco F Yoshida Fumiaki F Fellgiebel Andreas A Tomita Taisuke T Endres Kristina K Lichtenthaler Stefan F SF

EMBO molecular medicine 20190401 4

The metalloprotease ADAM10 is a drug target in Alzheimer's disease, where it cleaves the amyloid precursor protein (APP) and lowers amyloid-beta. Yet, ADAM10 has additional substrates, which may cause mechanism-based side effects upon therapeutic ADAM10 activation. However, they may also serve-in addition to APP-as biomarkers to monitor ADAM10 activity in patients and to develop APP-selective ADAM10 activators. Our study demonstrates that one such substrate is the neuronal cell adhesion protein ...[more]

PMID: 30833305

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LC-MS/MS analysis of isolated murine brain endothelial cells (BECs) from 3-, 6-, 12-, and 18-months-old mice and Apoe-KO mice at 3 months followed by label-free quantification (LFQ)

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Tetraspanin Tspan15 is an essential subunit of an ADAM10 scissor complex

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