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Co-IP (AP-MS) of Connexin-43 - Gap junction protein Connexin-43 is a direct transcriptional regulator of N-cadherin in vivo

ABSTRACT: Co-IP Experiment Bait: Cx43-Flag Cell line: Hela Related Publication: Gap Junction protein Connexin-43 is a direct transcriptional regulator of N-cadherin in vivo, Maria Kotini, Elias Barriga, Jonathan Leslie, Marc Gentzel, Verena Rauschenberger, Alexandra Schambony, and Roberto Mayor

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Marc Gentzel

LAB HEAD: Marc Gentzel

PROVIDER: PXD010870 | Pride | 2018-10-16

REPOSITORIES: Pride

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Gap junction protein Connexin-43 is a direct transcriptional regulator of N-cadherin in vivo.

Kotini Maria M Barriga Elias H EH Leslie Jonathan J Gentzel Marc M Rauschenberger Verena V Schambony Alexandra A Mayor Roberto R

Nature communications 20180921 1

Connexins are the primary components of gap junctions, providing direct links between cells under many physiological processes. Here, we demonstrate that in addition to this canonical role, Connexins act as transcriptional regulators. We show that Connexin 43 (Cx43) controls neural crest cell migration in vivo by directly regulating N-cadherin transcription. This activity requires interaction between Cx43 carboxy tail and the basic transcription factor-3, which drives the translocation of Cx43 t ...[more]

PMID: 30242148

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Gray but not white matter astroglia-specific connexin 43 ablation ameliorates neuroinflammation [Affymetrix]

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