Proteomics

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To invesigate the role of autophagy in Arabidopsis root cellular protein turnover and proteostasis

ABSTRACT: Protein turnover analysis using partial 15N labelling and protein abundance analysis using 15N spike-in were coupled to investiage the role of autophagy in protein degradation and proteostasis. Our findings show that dysfuncional autophagy due to loss-of-function autophagy components ATG5 and 11 lead to accumulation of protein targets with/without starvation stress. Further protein turnover analysis uncover a subgroup of proteins with retarded degradation. New selective autophagy protein targets involving glycolytic proteins were uncovered by this strategy. Furthermore, we found plasma membrane and cytoskeleton proteins show a general reduction, supporting the cross-interaction between autopahgy and vesicle trafficking pathway. Metabolomics changes in autophagy mutants due to a changed proteostasis was further investigated.

INSTRUMENT(S): 6520 Quadrupole Time-of-Flight LC/MS

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Root

SUBMITTER: Lei Li  

LAB HEAD: Harvey Millar

PROVIDER: PXD010900 | Pride | 2022-07-12

REPOSITORIES: Pride

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Publications

Defects in autophagy lead to selective in vivo changes in turnover of cytosolic and organelle proteins in Arabidopsis.

Li Lei L   Lee Chun Pong CP   Ding Xinxin X   Qin Yu Y   Wijerathna-Yapa Akila A   Broda Martyna M   Otegui Marisa S MS   Millar A Harvey AH  

The Plant cell 20220901 10

Identification of autophagic protein cargo in plants in autophagy-related genes (ATG) mutants is complicated by changes in protein synthesis and protein degradation. To detect autophagic cargo, we measured protein degradation rate in shoots and roots of Arabidopsis (Arabidopsis thaliana) atg5 and atg11 mutants. These data show that less than a quarter of proteins changing in abundance are probable cargo and revealed roles of ATG11 and ATG5 in degradation of specific glycolytic enzymes and of oth  ...[more]

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