Human Serum Immunoglobulin N-Glycosylation using nLC-MS/MS

ABSTRACT: Here we employ a nLC-MS/MS method to separate small amounts of purified immunoglobulins to characterize the N-glycan reportoire and site occupancy of bulk serum antibodies. Using this method, we have established, for the first time within individual donors, the N-linked glycan repotoire for bulk IgG1, IgG4, IgA1, IgA2 and IgM in healthy individuals. This is crucial for developing a platform to define disease-specific N-glycan signatures for different isotypes to help tune antibodes to induce protection.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

SUBMITTER: Kevin Chandler  

LAB HEAD: Catherine E Costello

PROVIDER: PXD010911 | Pride | 2019-01-24

REPOSITORIES: Pride