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Integrated transcriptomics and proteomics analyses reveal anthelmintic mechanism of arctigenin against Gyrodactylus kobayashii

ABSTRACT: The widespread resistance of parasitic worms to commonly used anthelmintics highlights the urgent need for development of new effective chemicals. Arctigenin (ARC) is a natural lignin compound that exhibits excellent anthelminthic efficacy against infections of Gyrodactylus, but knowledge about the mechanism of action is far from clear. In the present study, high-throughput RNA-sequencing was integrated with iTRAQ quantitative proteomics analysis to explore the anthelmintic mechanism and possible molecular targets of ARC against Gyrodactylus kobayashii.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Gyrodactylus Kobayashii Apis Mellifera (honeybee)

TISSUE(S): Whole Body

SUBMITTER: Xiao Tu

LAB HEAD: Gaoxue Wang

PROVIDER: PXD010913 | Pride | 2020-05-26

REPOSITORIES: Pride

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Proteome interrogation using gold nanoprobes to identify targets of arctigenin in fish parasites.

Tu Xiao X Tan Xiaoping X Qi Xiaozhou X Huang Aiguo A Ling Fei F Wang Gaoxue G

Journal of nanobiotechnology 20200218 1

Gold nanoparticles (GNPs) are one of the most widely used nanomaterials in various fields. Especially, the unique chemical and physical properties make them as the promising candidates in drug target identification, unfortunately, little is known about their application in parasites. In this paper, GNPs were employed as new solid support to identify drug targets of natural bioactive compound arctigenin (ARG) against fish monogenean parasite Gyrodactylus kobayashi. Before target identification, G ...[more]

PMID: 32070333

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