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SWATH-MS Analysis of Gfi1-mutant bone marrow neutrophils

ABSTRACT: This study investigates the proteomic alterations in bone marrow neutrophils isolated from 5-8 week old Gfi1+/-, Gfi1K403R/-, Gfi1R412X/-, and Gfi1R412X/R412X mice using the SWATH-MS technique. This dataset consists of 26 raw MS files, comprising 13 DDA (for spectral library generation)and 13 DIA (for SWATH) runs on a TripleTOF 5600 plus (SCIEX). Our findings revealed significant changes in the expression of neutrophil granule proteins and NADPH-oxidase complex components in Gfi1-mutant neutrophils.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Neutrophil, Bone Marrow

DISEASE(S): Neutropenia

SUBMITTER: H. Leighton Grimes

LAB HEAD: Kenneth D. Greis

PROVIDER: PXD010943 | Pride | 2020-04-07

REPOSITORIES: Pride

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Mouse models of neutropenia reveal progenitor-stage-specific defects.

Muench David E DE Olsson Andre A Ferchen Kyle K Pham Giang G Serafin Rachel A RA Chutipongtanate Somchai S Dwivedi Pankaj P Song Baobao B Hay Stuart S Chetal Kashish K Trump-Durbin Lisa R LR Mookerjee-Basu Jayati J Zhang Kejian K Yu Jennifer C JC Lutzko Carolyn C Myers Kasiani C KC Nazor Kristopher L KL Greis Kenneth D KD Kappes Dietmar J DJ Way Sing Sing SS Salomonis Nathan N Grimes H Leighton HL

Nature 20200422 7810

Advances in genetics and sequencing have identified a plethora of disease-associated and disease-causing genetic alterations. To determine causality between genetics and disease, accurate models for molecular dissection are required; however, the rapid expansion of transcriptional populations identified through single-cell analyses presents a major challenge for accurate comparisons between mutant and wild-type cells. Here we generate mouse models of human severe congenital neutropenia (SCN) usi ...[more]

PMID: 32494068

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