Dataset Information

“Overlapping pictures, proteomic study of the membrane components of signaling cascades in Botrytis cinerea controlled by phosphorylation”

ABSTRACT: The phytopathogen Botrytis cinerea is a ubiquitous fungus with a high capacity to adapt its metabolism to different hosts and environmental conditions in order to deploy a variety of virulence and pathogenicity factors and develop a successful plant infection. Here we report the first comparative phosphomembranome study of B. cinerea, aimed to analyze the membrane phosphoproteins composition of the fungus and its changes under different phenotypical conditions induced by two different carbon sources as plant based elicitors: glucose and deproteinized tomato cell wall (TCW). Proteomics analysisof membrane phosphoproteins was carried out by mass spectrometry. A total of 1115 proteins were successfully identified. Further analyses showed significant differences in the phosphomembranome composition depending on the available carbon source: 64 proteins were exclusively identified or overexpressed when the fungus was cultured with glucose as a sole carbon source, and 243 proteins were exclusively identified or overexpressed with TCW. GO and KEGG enrichment and clustering interaction analysis revealed changes in the composition of phosphomembranome with increase of autophagy in glucose conditions and protein degradation process, unfolded protein response (UPR) and ribosome biogenesis in TCW conditions.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Botryotinia Fuckeliana (strain B05.10) (noble Rot Fungus) (botrytis Cinerea)

TISSUE(S): Cell Culture, Fungal Cell

SUBMITTER: Francisco Fernández-Acero

LAB HEAD: Francisco Javier Fernández Acero

PROVIDER: PXD010961 | Pride | 2019-07-15

REPOSITORIES: Pride

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Publications

Proteomic study of the membrane components of signalling cascades of Botrytis cinerea controlled by phosphorylation.

Escobar-Niño Almudena A Liñeiro Eva E Amil Francisco F Carrasco Rafael R Chiva Cristina C Fuentes Carlos C Blanco-Ulate Barbara B Cantoral Fernández Jesús M JM Sabidó Eduard E Fernández-Acero Francisco Javier FJ

Scientific reports 20190708 1

Protein phosphorylation and membrane proteins play an important role in the infection of plants by phytopathogenic fungi, given their involvement in signal transduction cascades. Botrytis cinerea is a well-studied necrotrophic fungus taken as a model organism in fungal plant pathology, given its broad host range and adverse economic impact. To elucidate relevant events during infection, several proteomics analyses have been performed in B. cinerea, but they cover only 10% of the total proteins p ...[more]

PMID: 31285484

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Dataset Information

“Overlapping pictures, proteomic study of the membrane components of signaling cascades in Botrytis cinerea controlled by phosphorylation”

Publications

Proteomic study of the membrane components of signalling cascades of Botrytis cinerea controlled by phosphorylation.

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