Dataset Information

Real-time molecular diagnosis of tumors using water-assisted laser desorption/ionization mass spectrometry technology

ABSTRACT: This dataset contains MS imaging raw data as well as the SCiLS file used to confirm the regions in dog sarcoma biopsies classified by the SpiderMass system after analysis of the consecutive tissue section. All the tissues were gathered during dog sarcoma surgeries, snap-frozen and analysed later in the lab. The images were acquired on a MALDI LTQ orbitrap XL hybrid instrument.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Canis Familiaris (dog) (canis Lupus Familiaris)

TISSUE(S): Spleen, Gastrointestinal Tract, Mesenchymal Epithelium, Lung, Subcutaneous Tissue, Skin, Chorion, Bone

DISEASE(S): Sarcoma

SUBMITTER: Jusal Quanico

LAB HEAD: Michel Salzet

PROVIDER: PXD010990 | Pride | 2019-04-12

REPOSITORIES: Pride

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Publications

Real-Time Molecular Diagnosis of Tumors Using Water-Assisted Laser Desorption/Ionization Mass Spectrometry Technology.

Saudemont Philippe P Quanico Jusal J Robin Yves-Marie YM Baud Anna A Balog Julia J Fatou Benoit B Tierny Dominique D Pascal Quentin Q Minier Kevin K Pottier Mélissa M Focsa Cristian C Ziskind Michael M Takats Zoltan Z Salzet Michel M Fournier Isabelle I

Cancer cell 20181018 5

Histopathological diagnosis of biopsy samples and margin assessment of surgical specimens are challenging aspects in sarcoma. Using dog patient tissues, we assessed the performance of a recently developed technology for fast ex vivo molecular lipid-based diagnosis of sarcomas. The instrument is based on mass spectrometry (MS) molecular analysis through a laser microprobe operating under ambient conditions using excitation of endogenous water molecules. Classification models based on cancer/norma ...[more]

PMID: 30344004

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Project description:Aberrant protease activity has been implicated in the etiology of various prevalent diseases including neurodegeneration and cancer, in particular metastasis. Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) has recently been established as a key technology for bioanalysis of multiple biomolecular classes such as proteins, lipids, and glycans. However, it has not yet been systematically explored for investigation of a tissue’s endogenous protease activity. In this study, we demonstrate that different tissues, spray-coated with substance P as a tracer, digest this peptide with different time-course profiles. Furthermore, we reveal that distinct cleavage products originating from substance P are generated transiently and that proteolysis can be attenuated by protease inhibitors in a concentration-dependent manner. To show the translational potential of the method, we analyzed protease activity of gastric carcinoma in mice. Our MSI and quantitative proteomics results reveal differential distribution of protease activity – with strongest activity being observed in mouse tumor tissue, suggesting the general applicability of the workflow in animal pharmacology and clinical studies.Aberrant protease activity has been implicated in the etiology of various prevalent diseases including neurodegeneration and cancer, in particular metastasis. Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) has recently been established as a key technology for bioanalysis of multiple biomolecular classes such as proteins, lipids, and glycans. However, it has not yet been systematically explored for investigation of a tissue’s endogenous protease activity. In this study, we demonstrate that different tissues, spray-coated with substance P as a tracer, digest this peptide with different time-course profiles. Furthermore, we reveal that distinct cleavage products originating from substance P are generated transiently and that proteolysis can be attenuated by protease inhibitors in a concentration-dependent manner. To show the translational potential of the method, we analyzed protease activity of gastric carcinoma in mice. Our MSI and quantitative proteomics results reveal differential distribution of protease activity – with strongest activity being observed in mouse tumor tissue, suggesting the general applicability of the workflow in animal pharmacology and clinical studies.

Dataset Information

Real-time molecular diagnosis of tumors using water-assisted laser desorption/ionization mass spectrometry technology

Publications

Real-Time Molecular Diagnosis of Tumors Using Water-Assisted Laser Desorption/Ionization Mass Spectrometry Technology.

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