Proteomics

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Quantitative (Glyco-)Proteomics of Haloferax volcanii Biofilm Formation

ABSTRACT: A quantitative proteomics analysis comparing planktonic and sessile cells at different stages of biofilm development, using iTRAQ labeling, has been performed for Haloferax volcanii. Samples were taken from liquid, shaking cultures were compared to samples from static cultures, separated into sessile (biofilm) and planktonic cells. This dataset resulted in the identification of more than 50% of the H. volcanii proteome. Most importantly, expression as well as N-glycosylation of various surface filaments (pili, flagella and SLG) has been quantified, strongly supporting the hypothesis, that differential N-glycosylation plays important roles in the biofilm formation of H. volcanii.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Haloferax Volcanii (halobacterium Volcanii)

SUBMITTER: Stefan Schulze  

LAB HEAD: Mechthild Pohlschroder

PROVIDER: PXD011012 | Pride | 2020-05-11

REPOSITORIES: Pride

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While many aspects of archaeal cell biology remain relatively unexplored, systems biology approaches like mass spectrometry (MS) based proteomics offer an opportunity for rapid advances. Unfortunately, the enormous amount of MS data generated often remains incompletely analyzed due to a lack of sophisticated bioinformatic tools and field-specific biological expertise for data interpretation. Here we present the initiation of the Archaeal Proteome Project (ArcPP), a community-based effort to comp  ...[more]

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